Interleukin-12 alpha / IL12A Mouse Protein

Specifications

Product Data
Species	Mouse
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Predicted MW	75 kDa
Purity	>98% by SDS-PAGE and HPLC analyses
Buffer	Presentation State: Purified State: Lyophilized purified protein Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40kDa (p40) subunits. AA sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS Endotoxin Levels: < 0.1 ng/µg of protein (<1EU/µg)
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Assay #1: The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml (> 1 x 107 units/mg). Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤0.1ng/ml (≥1 x 107 units/mg).
Reference Data
RefSeq	NP_001152896
Locus ID	16159
UniProt ID	P43431, F8WI71
Cytogenetics	3 31.92 cM
Synonyms	Il-12a; IL-12p35; Ll12a; p35
Summary	Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.[UniProtKB/Swiss-Prot Function]

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