UCHL1 / PGP9.5 (1-223) (recombinant) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
Predicted MW	24.8 kDa
Concentration	lot specific
Purity	>95% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl pH 8.0, 2 mM EDTA
Preparation	Liquid purified protein
Applications	Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC (Enzo, Cat.No, BML-SE211) - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077) - Fluorescent plate reader (PerkinElmer, VICTOR X3)
Protein Description	Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific activity is > 150pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
Reference Data
RefSeq	NP_004172
Locus ID	7345
UniProt ID	P09936, V9HW74
Cytogenetics	4p13
Synonyms	HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1
Summary	The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Protein Families	Druggable Genome, Protease
Protein Pathways	Parkinson's disease

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