CNTF (1-200, His-tag) Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM
Tag	His-tag
Predicted MW	25.2 kDa
Concentration	lot specific
Purity	>95% by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: Phosphate buffered saline (pH 7.4) containing 10% glycerol.
Preparation	Liquid purified protein
Protein Description	Recombint rat CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less or equal to 40 ng/ml.
Endotoxin	< 1.0 EU per 1 microgram of protein (determined by LAL method)
Reference Data
RefSeq	NP_037298
Locus ID	25707
UniProt ID	P20294
Cytogenetics	1q43
Summary	a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD, Feb 2006]

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