Hepatitis B Surface Antigen / HBsAg (adw) Protein

Specifications

Product Data
Expression Host	S. cerevisiae
Concentration	lot specific
Purity	>98% pure by SDS-PAGE. Purification is conducted from broken yeast cells by the following scheme: Clarification, Microfiltration, Ultrafiltration, Adsorption Chromatography, Ion-exchange chromatography, Ultracentrifugation and Sterile Filtration
Buffer	Presentation State: Purified State: Liquid (sterile filtered) purified protein Buffer System: 0.05M Phosphate, 0.2M Sodium Chloride, pH 7.2 without preservatives
Preparation	Liquid (sterile filtered) purified protein
Applications	Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.
Protein Description	Recombinant Hepatitis B Surface Antigen (HBsAg), (adw) (S region) is a 24 kDa protein containing 227 amino acids, comprising the full-length of the S-gene. Does not contain a fusion partner.
Storage	Store the antigen at 2-8°C. DO NOT FREEZE!!
Stability	Shelf life: six months from despatch.
Reference Data
Summary	Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

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