Tumor necrosis factor (TNF-alpha) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	17.48 kDa
Concentration	N/A
Purity	>98% by RP-HPLC, Anion-exchange FPLC, Silver stain
Buffer	Presentation State: Purified State: Lyophilized purified fraction Buffer System: 20 mM PBS, pH 7.2, 10 mM NaCl
Preparation	Lyophilized purified fraction
Protein Description	Recombinant Human TNF-a produced in E. coli is a single, non-glycosylated polypeptide having a Molecular Mass of 17.4 kDa protein and consisting of 158 amino acid residues.
Storage	Store lyophilized rh TNF-alpha desiccated at -20°C. Reconstituted rh TNF-alpha should be stored in working aliquots at -18°C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid repeated freezing and thawing.
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: Human rh TNF-alpha is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of Murine L929 cells in the presence of Actinomycin-D is < 0.05 ng/ml. Specific: 2 x 107 units/mg
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh TNF-alpha
Reference Data
RefSeq	NP_000585
Locus ID	7124
UniProt ID	P01375, Q5STB3
Cytogenetics	6p21.33
Synonyms	DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

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