Protein G Protein

Specifications

Product Data
Purity	This product is chromatographically pure Protein G and shows predominantly a single band by SDS-PAGE.
Buffer	Presentation State: Aff - Purified State: Lyophilized purified Ig fraction without preservatives.
Preparation	Lyophilized purified Ig fraction without preservatives.
Applications	Suitable for use as an antigen, as a Control or standard in assays, for conjugation and for most other immunological methods. This recombinant Protein G contains only IgG binding domains, ensuring maximum specific IgG binding.
Protein Description	Protein G (recombinant, E. coli), unconjugated. Extinction Coefficient: 1% at 280 nm is 9.5 (E0.1% of 9.5). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Protein G. No reaction was observed against anti-Protein A.
Note	Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3.
Storage	Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

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