Tumor necrosis factor (TNF-alpha) Bovine Protein

Specifications

Product Data
Species	Bovine
Expression Host	Pichia pastoris
Predicted MW	17.4 kDa
Purity	>95% pure by SDS PAGE analysis
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS. Further dilutions should be made in a buffer containing carrier protein. Preservative: None Stabilizer: None
Preparation	Lyophilized purified protein
Applications	ELISA: SA1000 May be used as a standard with SM2216P or SM2228B. Functional Assays.
Protein Description	Recombinant Bovine TNF alpha expressed in Pichia pastoris.
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: ED50 is in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant Bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
Endotoxin	< 0.05 EU/µg
Reference Data
RefSeq	NP_776391
Locus ID	280943
UniProt ID	Q06599
Cytogenetics	23q22
Synonyms	TNF-a; TNF-alpha; TNFa
Summary	tumor necrosis factor

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