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Aminoacylase 1 (ACY1) (NM_000666) Human Recombinant Protein
CAT＃: TP301284
Recombinant protein of human aminoacylase 1 (ACY1), 20 µg
View other "Aminoacylase 1" proteins (10)
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC201284 protein sequence
Red=Cloning site Green=Tags(s) MTSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNP TLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIH MTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRF MEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPD VDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNR YIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 45.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_000657 |
| Locus ID | 95 |
| UniProt ID | Q03154, V9HWA0 |
| Refseq Size | 1678 |
| Cytogenetics | 3p21.2 |
| Refseq ORF | 1224 |
| Synonyms | ACY-1; ACY1D; HEL-S-5 |
| Summary | This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| Protein Families | Protease |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC424578 | ACY1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC434169 | ACY1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC434175 | ACY1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC434192 | ACY1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY424578 | Transient overexpression lysate of aminoacylase 1 (ACY1) |
CNY 3,080.00 |
|
| LY434169 | Transient overexpression lysate of aminoacylase 1 (ACY1), transcript variant 3 |
CNY 3,080.00 |
|
| LY434175 | Transient overexpression lysate of aminoacylase 1 (ACY1), transcript variant 4 |
CNY 3,080.00 |
|
| LY434192 | Transient overexpression lysate of aminoacylase 1 (ACY1), transcript variant 5 |
CNY 3,080.00 |
|
| PH301284 | ACY1 MS Standard C13 and N15-labeled recombinant protein (NP_000657) |
CNY 19,520.00 |
|
| TP710321 | Purified recombinant protein of Human aminoacylase 1 (ACY1), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
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