Ifng (NM_008337) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Mouse IFN-gamma;, the region of His23-Cys155, from gene Accession# NM_008337
Tag	Tag Free
Predicted MW	15.7 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	20 mM HEPES, 150 mM NaCl, 5 mM TCEP; pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.2-0.6 ng/ml, corresponding to a specific activity of 5 - 1.67 x 10^6 units/mg, determined by inhibition of WEHI-279 cell proliferation induced by mouse IFNγ.
Endotoxin	Less than 0.01 ng per ug protein as determined by the LAL method
Reference Data
RefSeq	NP_032363
Locus ID	15978
UniProt ID	P01580
Refseq Size	1207
Cytogenetics	10 66.75 cM
Refseq ORF	465
Synonyms	If; If2f; Ifg; IFN-g
Summary	This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

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