Mmp9 (20-730, His-Tag) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) APYQRQPTFV VFPKDLKTSN LTDTQLAEAY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS QTLKAIRTPR CGVPDVGRFQ TFKGLKWDHH NITYWIQNYS EDLPRDMIDD AFARAFAVWG EVAPLTFTRV YGPEADIVIQ FGVAEHGDGY PFDGKDGLLA HAFPPGAGVQ GDAHFDDDEL WSLGKGVVIP TYYGNSNGAP CHFPFTFEGR SYSACTTDGR NDGTPWCSTT ADYDKDGKFG FCPSERLYTE HGNGEGKPCV FPFIFEGRSY SACTTKGRSD GYRWCATTAN YDQDKLYGFC PTRVDATVVG GNSAGELCVF PFVFLGKQYS SCTSDGRRDG RLWCATTSNF DTDKKWGFCP DQGYSLFLVA AHEFGHALGL DHSSVPEALM YPLYSYLEGF PLNKDDIDGI QYLYGRGSKP DPRPPATTTT EPQPTAPPTM CPTIPPTAYP TVGPTVGPTG APSPGPTSSP SPGPTGAPSP GPTAPPTAGS SEASTESLSP ADNPCNVDVF DAIAEIQGAL HFFKDGWYWK FLNHRGSPLQ GPFLTARTWP ALPATLDSAF EDPQTKRVFF FSGRQMWVYT GKTVLGPRSL DKLGLGPEVT HVSGLLPRRL GKALLFSKGR VWRFDLKSQK VDPQSVIRVD KEFSGVPWNS HDIFQYQDKA YFCHGKFFWR VSFQNEVNKV DHEVNQVDDV GYVTYDLLQC P
Tag	His-Tag
Predicted MW	79.3kDa (717aa)
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl (pH 7.5) containing 1mM CaCl2, 100mM NaCl, 10% glycerol
Applications	SDS-PAGE, Enzyme Activity
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
Bioactivity	Specific activity is >1,500 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_038627.1
Summary	MMP-9, also known matrix metalloproteinase-9, is one of the matrix metalloproteinases superfamily which is zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. It degrades many substrates such as gelatin, collagens, elastin and proteoglycan core protein which appears to be involved in invasive ability. This protein also plays an essential role in leukocyte migration and in bone osteoclastic resorption. It plays an important role in angiogenesis and neovascularization and so appears to be involved in the remodeling associated with malignant glioma neovascularization. Recombinant mouse MMP-9 protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

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