PRSS3 (16-247, His-Tag) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) VPFDDDDKIV GGYTCEENSL PYQVSLNSGS HFCGGSLISE QWVVSAAHCY KTRIQVRLGE HNIKVLEGNE QFINAAKIIR HPKYNRDTLD NDIMLIKLSS PAVINARVST ISLPTAPPAA GTECLISGWG NTLSFGADYP DELKCLDAPV LTQAECKASY PGKITNSMFC VGFLEGGKDS CQRDSGGPVV CNGQLQGVVS WGHGCAWKNR PGVYTKVYNY VDWIKDTIAA NS
Tag	His-Tag
Predicted MW	26kDa (238aa)
Concentration	1mg/ml (determined by Absorbance at 280nm)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Applications	SDS-PAGE, Enzyme Activity
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity	Specific activity is > 10,000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 per minute at pH 8.0 at 37℃.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_002762.2
Summary	PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. It has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins. Recombinant human Trypsin 3/PRSS3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

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