PGF (19-149, His-Tag) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERCGDAVPR R
Tag	His-Tag
Predicted MW	15.5kDa (137aa)
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Applications	SDS-PAGE, Bioactivity
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity	Measured by its binding ability in a functional ELISA with Human VEGFR1/Flt-1 (CAT# ATGP4101).
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_001193941.1
Summary	Placenta growth factor (PlGF/PGF) is a member of the PDGF/VEGF-sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. There are three isoforms of this protein: PlGF-1, PlGF-2, PlGF-3. PlGF-2 is only found in early placenta up until the 8th week of development, while PlGF-1 is specifically found in the colon as well as mammary carcinomas. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. Also, PlGF is ultimately associated with angiogenesis. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Recombinant human PlGF-1/PGF, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

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