Lif (NM_008501) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence from TrueORF clone, MR223120, encoding the domain (Ser24-Phe203) of mouse Lif
Tag	Tag Free
Predicted MW	19.9 kDa
Concentration	Resuspend the protein to the desired concentration in proper buffer.
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 20 mM PB, pH 7.4
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Measured by its ability to induce differentiation of M1 cells. ED50 for this effect is typically 0.20-0.30 ng/ml.
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_032527
Locus ID	16878
UniProt ID	P09056
Refseq Size	4015
Cytogenetics	11 2.94 cM
Refseq ORF	609
Summary	LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.[UniProtKB/Swiss-Prot Function]

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