TDT (Dntt) (NM_001043228) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) 1        MHHHHHHSSP SPVPGSQNVP APAVKKISQY ACQRRTTLNN YNQLFTDALD ILAENDELRE 61       NEGSCLAFMR ASSVLKSLPF PITSMKDTEG IPCLGDKVKS IIEGIIEDGE SSEAKAVLND 121      ERYKSFKLFT SVFGVGLKTA EKWFRMGFRT LSKIQSDKSL RFTQMQKAGF LYYEDLVSCV 181      NRPEAEAVSM LVKEAVVTFL PDALVTMTGG FRLGKMTGHD VDFLITSPEA TEDEEQQLLH 241      KVTDFWKQQG LLLYCDILES TFEKFKQPSR KVDALDHFQK CFLILKLDHG RVHSEKSGQQ 301      EGKGWKAIRV DLVMCPYDRR AFALLGWTGS PQFLRDLRRY ATHERKMMLD NHALYDRTKR 361      VFLEAESEEE IFAHLGLDYI EPWERNA
Tag	N-His
Predicted MW	44.3 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001036693
Locus ID	21673
UniProt ID	P09838, Q3UZ80
Cytogenetics	19 34.32 cM
Synonyms	Tdt
Summary	Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function]

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