pCas-Guide-EF1a-RFP

CAT#: GE100140

pCas-Guide-EF1a-RFP vector (with Cas9 and RFP expression) for genomic target sequence cloning (10 µg)



CNY 5,020.00


货期*
现货

规格
    • 10 ug

Product images

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • All-in-one vector
  • Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
  • BamH I and BsmB I sites are used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • EF1a promoter driven RFP to enrich transfected cells
  • CF3 primer (GE100008) can be used to sequence the target sequence after cloning
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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