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Mouse IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA : 1:20,000 - 1:100,000 Western Blot : 1:2,000 - 1:10,000 Immunohistochemistry: 1:1,000 - 1:5,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Secondary Antibodies
Mouse IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain) chicken polyclonal antibody, TRITC
| Applications | Immunomicroscopy. Flow Cytometry or FACS analysis. |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG (H+L chain) chicken polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Mouse |
| Conjugation | Texas Red |
Mouse IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain) chicken polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H+L chain) chicken polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the stated concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | AP |
