Secondary Antibodies

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) chicken polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based Fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
IF Microscopy: 1/500-1/2,500.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation Texas Red

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/140,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) chicken polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,800 to 1:25,800 of the stated concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (H+L chain) chicken polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:3,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation Biotin