Secondary Antibodies

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Goat IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1500 - 1:2,500
ELISA: 1:5,000 - 1:25,000
Conjugation HRP

Human IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:500 - 1:5,000
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Rabbit IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:100,000
Reactivities Rabbit
Conjugation HRP

Mouse IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 to 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:70,000
Reactivities Mouse
Conjugation HRP

Sheep IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:3,000 - 1:15,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000 - 1:150,000
Conjugation HRP

Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:65,000
Conjugation HRP

Rabbit IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:3,000
IHC: 1:500 - 1:2,000
ELISA: 1:40,000
Reactivities Rabbit
Conjugation HRP

Rat IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Chicken IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:65,000
Conjugation HRP

Goat IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:150,000
Reactivities Goat
Conjugation HRP

Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
ELISA: 1:10,000 - 1:50,000
Conjugation HRP