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Secondary Antibodies
Canine IgG (H+L chain) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Human IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
| Reactivities | Human |
| Conjugation | HRP |
Duck IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000. Immunohistochemistry and Cytochemistry: 1/50-1/250. Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | HRP |
Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rat |
| Conjugation | Biotin |
Goat Monoclonal Anti-Rabbit IgG Fab antibody, clone RMG01
| Applications | ELISA: 0.01ug/mL – 0.5ug/mL; ICC/IHC: 0.5ug/mL-2ug/mL; WB (non-reduced): 0.1ug/mL-0.5ug/mL |
| Reactivities | Rabbit IgG Fab |
| Conjugation | Unconjugated |
Goat Polyclonal Anti-Human IgG Antibody
| Applications | WB 1:500-1:2,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG2b Antibody Rhodamine Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 ELISA: 1:2,000 - 1:10,000 FLISA: 1:20,000 - 1:100,000 |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
