Secondary Antibodies

Porcine IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Porcine
Conjugation HRP

Swine IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Porcine
Conjugation Unconjugated

Swine IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:100,000 - 1:200,000
Reactivities Porcine
Conjugation HRP

Swine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:10,000
Reactivities Porcine
Conjugation Alkaline Phosphatase

Porcine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used:
• In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
• To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
• As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation Biotin

Porcine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used:
• In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen using a reference antibody of swine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation HRP

Porcine IgG F(c) specific goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Western Blot: 1/1000-1/30,000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2000.
Reactivities Porcine
Conjugation FITC

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/10000.
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP

Porcine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Porcine
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Porcine
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP

Porcine IgG F(c) specific goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000.
Reactivities Porcine
Conjugation Biotin

Porcine IgA goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin