Secondary Antibodies

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Rabbit IgG (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/110,000.
Western Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation HRP

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Mouse IgA goat polyclonal antibody, HRP

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/4000 - 1/8000.
Western Blot.
Reactivities Mouse
Conjugation HRP

Mouse IgG (Rat adsorbed) goat polyclonal antibody, Aff - Purified

Applications ELISA.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000.
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP

Rat IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1:10000.
Western Blot: 1:1000 - 1:10000.
Reactivities Rat
Conjugation HRP

Mouse IgA (+IgG +IgM) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgE goat polyclonal antibody, HRP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation HRP

Rabbit IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG F(ab')2 specific goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation TRITC