Secondary Antibodies

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immonoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremly low background levels, absence of F(c) mediated binding, lot-to-lot consitency, high titer and specificy.
ELISA: 1:82,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:500 - 1:2,500.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Biotin

Applications Suitable for Western Blot, ELISA,
Immunohistochemistry, Immunomicroscopy, as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:250,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation Biotin

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/300,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Human
Conjugation HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000-1/50,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Rat IgA (alpha chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Mouse
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy (1:1,000-1:5,000) and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
 ELISA : 1/10,000-1/50,000.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation Biotin

Rat IgM (Mu chain specific) goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities Rat
Conjugation PE

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation Texas Red

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
ELISA: 1/10,000 - 1/50,000.
Western Blot: 1/2,000 - 1/5,000.
Immunohistochemistry: 1/1,000 - 1/3,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:250) and Flow cytometry (1:100-1:250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rat
Conjugation PE

Rat IgG (H+L chain) F(ab')2 Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/22,000
Western Blot: User optimized.
Immunohistochemistry: User optimized.
Reactivities Rat
Conjugation Unconjugated

Mouse IgM (Mu chain specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.
Recommended dilutions:
Flow cytometry 1:100 - 1:250
IF microscopy 1:100 - 1:250
Reactivities Mouse
Conjugation PE