Secondary Antibodies

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:7,500 to 1:32,000 is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (Fc specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:600 to 1:2,800 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Mouse IgA (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:6,300
Western Blot: 1:200 - 1:1,000
Immunohistochemistry: 1:100 - 1:500
Reactivities Mouse
Conjugation AP

Human IgA + IgG + IgM goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western blot: 1/500-1/3,000, dot blot), ELISA (1/2,000-1/10,000) and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation AP

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation AP

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,500 of the reconstitution concentration is suggested for this product.
Reactivities Hamster
Conjugation AP

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA: 1:1,000 - 1:5,000
Western blot: 1:500 - 1:5,000
Immunohistochemistry: 1:200 - 1:1,000.
Reactivities Rat
Conjugation AP

Rat IgA (alpha chain) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Rat IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:8,000 is suggested for this product.
Reactivities Rat
Conjugation AP

Rabbit IgG (F(ab')2 specific, adsorbed) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,100 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (Fc specific, adsorbed) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western: 1/500-1/2,500 or Dot blot), ELISA (1/2,000-1/10,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications ELISA: 1:9,600.
Western blot: 1:500 - 1:2,500.
Immunohistochemistry: 1:200 - 1:1,000.
Reactivities Mouse
Conjugation AP

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP