Secondary Antibodies

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Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049BT-N

CNY 5,640.00

5周

Porcine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used:
• In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
• To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
• As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation Biotin
F(ab')2 Human IgA (alpha chain) Antibody Biotin Conjugated
TA398163

CNY 4,988.00

4周

F(ab')2 Human IgA (alpha chain) Antibody Biotin Conjugated

Applications WB: 1:20,000 - 1:400,000
IHC: 1:500 - 1:5,000
IF: 1:200 - 1:1,000
FC: 1:200 - 1:1,000
ELISA: 1:20,000 - 1:400,000
Conjugation Biotin
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447BT-N

CNY 7,710.00

5周

Duck IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunohistochemistry and Immunocytochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation Biotin
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138BT-N

CNY 1,930.00

4周

Turkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Reactivities Turkey
Conjugation Biotin
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543BT-N

CNY 5,640.00

5周

Mouse IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
For histochemical and Cytochemical use are usually between 1/100 and 1/250
In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivities Mouse
Conjugation Biotin
Human IgA (alpha chain) Goat Polyclonal Antibody
AP00640BT-N

CNY 3,245.00

4周

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin
Human IgA + IgG + IgM Goat Polyclonal Antibody
R1343B

CNY 3,188.00

4周

Human IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Western Blot: 1/5000-1/20000.
ELISA: 1/400000.
Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000.
Reactivities Human
Conjugation Biotin
Mouse IgG2b Antibody Biotin Conjugated Pre-adsorbed
TA397835

CNY 3,713.00

4周

Mouse IgG2b Antibody Biotin Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG F(ab')2 Antibody Biotin Conjugated
TA397897

CNY 3,356.00

4周

Mouse IgG F(ab')2 Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin
Mouse IgG3 Antibody Biotin Conjugated Pre-adsorbed
TA397836

CNY 3,713.00

4周

Mouse IgG3 Antibody Biotin Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG2a Antibody Biotin Conjugated Pre-adsorbed
TA397834

CNY 3,713.00

4周

Mouse IgG2a Antibody Biotin Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin
Mouse IgM (mu chain) Antibody Biotin Conjugated
TA397898

CNY 3,938.00

4周

Mouse IgM (mu chain) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:10,000 - 1:50,000
Conjugation Biotin
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody
AP33463BT-N

CNY 4,169.00

4周

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Biotin, Aff - Purified

Applications Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020BS

CNY 4,279.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Western Blot.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Rat
Conjugation Biotin
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795BT-N

CNY 4,026.00

5周

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications ELISA.
Western Blot (1/5000).
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate.
Reactivities Chicken
Conjugation Biotin