Secondary Antibodies

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Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491BT-N

CNY 5,640.00

5周

Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation Biotin
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483BT-N

CNY 5,640.00

5周

Rat IgE (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493BT-N

CNY 5,640.00

5周

Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used:
In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user's choice has to be used.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000.
Reactivities Rat
Conjugation Biotin
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020BS

CNY 4,279.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Western Blot.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Rat
Conjugation Biotin
Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
R1377B

CNY 3,938.00

5周

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/150,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin
Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody
R1414B

CNY 3,713.00

4周

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, Biotin

Applications Suiatble for use Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:90,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin
Rat IgA (alpha chain) Goat Polyclonal Antibody
R1376B

CNY 4,031.00

4周

Rat IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin
Rat IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody
R1391B

CNY 3,938.00

4周

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Immunoblotting
ELISA
Immunohistochemistry
Immunomicroscopy
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:110,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
R1378B

CNY 3,994.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (1/2,000-1/10,000), ELISA, Immunohistochemistry (1/1,000-1/5,000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin