Secondary Antibodies

Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
IF Microscopy.
ELISA: 1/10,000-1/60,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2500.
Immunoprecipitation.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H&L) Antibody

Applications WB: 1:5,000 - 1:25,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA: 1/15,000-1/30,000
Western blot: 1/500-1/2,000
Immunohistochemistry: 1/500-1/2,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/3000.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG F(ab')2 specific goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA 1:30,000
Western Blot: 1/5,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation Unconjugated

F(ab')2 Rabbit IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (Fc specific, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1:20,000 - 1:100,000.
Western Blot: 1:2,000 - 1:10,000.
Immunohistochemistry: 1:1,000 - 1:5,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA 1:10,000-1:50,000
Western blot 1:1,000-1:5,000
Immunohistochemistry 1:500-1:3,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilution for ELISA: 1:4,000 - 1:20,000.
Reactivities Rabbit
Conjugation Unconjugated