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PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose
| Applications | Immunoprecipitation. |
| Reactivities | Chicken |
| Conjugation | Agarose |
Secondary Antibodies
Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgM goat polyclonal antibody, HRP
| Applications | ELISA (1/10,000-1/100,000). |
| Reactivities | Chicken |
| Conjugation | HRP |
Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Chicken |
| Conjugation | HRP |
Chicken IgA goat polyclonal antibody, HRP
| Applications | ELISA (1/10,000-1/100,000). |
| Reactivities | Chicken |
| Conjugation | HRP |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC
| Applications | Immunocytochemistry (1/200). In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Conjugation | FITC |
Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Chicken |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
| Reactivities | Chicken |
| Conjugation | FITC |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Chicken |
| Conjugation | TRITC |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Texas Red
| Applications | Suitable for Imunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Chicken |
| Conjugation | Texas Red |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
| Applications | Suitable for immunoblotting (western or dot blot, 1:500- 1:2,000), ELISA (1:2,000-1:8,000) and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. |
| Reactivities | Chicken |
| Conjugation | AP |
Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, TRITC
| Applications | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Chicken |
| Conjugation | TRITC |
Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Chicken |
| Conjugation | Texas Red |
