Secondary Antibodies

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation.
Reactivities Chicken
Conjugation Unconjugated

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA.
Second step reagent for rat IgG2a Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin