Secondary Antibodies

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), FITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect Immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, FITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), FITC

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), FITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), FITC

Applications To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), FITC

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgD mouse monoclonal antibody, clone NI 158, FITC

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgD and cell membrane IgD.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
Reactivities Human
Conjugation FITC

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), FITC

Applications To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), FITC

Applications To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA2, and Immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation FITC

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), FITC

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), FITC

Applications To identify the presence of IgA1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA1, and Immunoblotting
using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC