Secondary Antibodies

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Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485BT-N

CNY 7,161.00

5周

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253P

CNY 2,520.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/400,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
TA397920

CNY 1,744.00

4周

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:200,000 - 1:300,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
TA397921

CNY 1,219.00

4周

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:300,000
Reactivities Mouse
Conjugation Biotin
Mouse IgM (mu chain) Antibody Peroxidase Conjugated
TA397912

CNY 4,200.00

4周

Mouse IgM (mu chain) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:180,000
Reactivities Mouse
Conjugation HRP
Mouse IgM (H&L) Antibody Peroxidase Conjugated
TA397267

CNY 9,038.00

4周

Mouse IgM (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation HRP
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992AF-N

CNY 6,260.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used: 
As unlabelled primary or secondary antibody reagent for indirect detection of IgM in Mouse cells, tissues and body fluids in Immunofluorescence and Immunoenzyme assay methods.
For the production of immunoconjugates with a selected marker.
To prepare immunoaffinity adsorbents by coupling to an artificial carrier.
In non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent.
In Western blotting.
When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before.
Recommneded Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. 
Precipitin titre: not less than 1/64 when tested against normal Mouse serum in agar-block immunodiffusion titration. 

Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (Fc specific) Rabbit Polyclonal Antibody
R1352P

CNY 2,657.00

4周

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse lambda light chain Rabbit Polyclonal Antibody
R1358HRP

CNY 4,650.00

4周

Mouse lambda light chain rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/50,000 .
Western Blot: 1/1,000 - 1/5,000.
Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
Reactivities Mouse
Conjugation HRP
Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody
R1360HRP

CNY 4,369.00

4周

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253B

CNY 2,877.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253AP

CNY 3,224.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Mouse IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
R1350P

CNY 2,963.00

4周

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:40,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Biotin Conjugated
TA397908

CNY 3,713.00

4周

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin
Mouse IgM (mu chain) Antibody
TA397911

CNY 956.00

4周

Mouse IgM (mu chain) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Unconjugated