Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA : 1:50,000 - 1:300,000 Western Blot : 1:10,000 - 1:30,000 Immunohistochemistry: 1:500 - 1:2,500 Other Dilution: User Optimized |
| Reactivities | Mouse |
| Conjugation | HRP |
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
| Reactivities | Goat |
| Conjugation | HRP |
Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for ELISA, Immunofluorescence Microscopy. Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1000-1/5000. |
| Reactivities | Mouse |
| Conjugation | FITC |
Cy3 Conjugated Rabbit Anti-Goat IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Goat IgG |
| Conjugation | Cy3 |
Biotin Conjugated Rabbit Anti-human IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Human IgG |
| Conjugation | Biotin |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/500,000. Western blot: 1/2,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Chicken |
| Conjugation | Biotin |
Mouse lambda light chain rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Monkey IgG (Fab specific) rabbit polyclonal antibody, TRITC
| Applications | Can be used for direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. |
| Reactivities | Monkey |
| Conjugation | TRITC |
Human IgG rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP
| Applications | Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays. Recommended Dilutions: ELISA: 1/300,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG rabbit polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | HRP |
Goat IgM rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
Goat IgA rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
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