Secondary Antibodies

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000.
Western Blot: 1:2,000-1:10,000.
Immunohistochemistry: 1:1,000-1:5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation FITC

Rat IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation FITC

Rat IgG Fc specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation TRITC

Rat IgG Fc specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Western Blot: 1:2,000-1:10,000.
Immunohistochemistry: 1:1,000-1:5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Dot blot or Western blot, 1/1000-1/4,000), ELISA (1/2,000-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation AP

Rat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation TRITC

Rat IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Rat
Conjugation FITC

Rat IgM (Mu chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/70,000 of the concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin

Rat IgG F(ab)2 specific rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgM (Mu chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Fluorescence microscopy and Flow cytometry.
Reactivities Rat
Conjugation Texas Red

Rat IgG Fc specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red