Secondary Antibodies

Rat IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Dot blot or Western blot, 1/1000-1/4,000), ELISA (1/2,000-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation AP

Rat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation TRITC

Rat IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Rat
Conjugation FITC

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
ELISA: 1/20000 - 1/100000.
Western Blot: 1/2000 - 1/10000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Sheep IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation Texas Red

Sheep IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Sheep
Conjugation TRITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
Reactivities Sheep
Conjugation Biotin

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Texas Red

Applications FLISA: 1/10000 - 1/50000.
Immunofluorescence: 1/1000 - 1/5000.
Flow Cytometry: 1/500 - 1/2500.
Reactivities Pig
Conjugation Texas Red

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
Flow Cytometry: 1:2,000-1:10,000.
IF Microscopy: 1:500-1:2,500.
Reactivities Pig
Conjugation FITC

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:120,000 of the reconstitution concentration is suggested for this product.
Reactivities Pig
Conjugation Biotin

Porcine IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000.
Western Blot: 1:2,000-1:10,000.
Immunohistochemistry: 1:1,000-1:5,000.
Reactivities Pig
Conjugation Unconjugated