Secondary Antibodies

Rat IgG F(ab)2 specific rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:6,000 to 1:25,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin

Rat IgG Fc specific rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation TRITC

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilutions:
FACS: 1/2,000-1/10,000.
IF microscopy: 1/500-1/2,500.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG2b in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:75,000 is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy, Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation TRITC

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Texas Red

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation TRITC

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Texas Red

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/8,000-1/32,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
Flow Cytometry: 1/500-1/2,500.
Immunofluorescence microscopy: 1/500-1/2,500.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
IF Microscopy: 1/500-1/2,500.
Reactivities Mouse
Conjugation Texas Red

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
IF microscopy 1:500 - 1:2,500
Reactivities Mouse
Conjugation FITC

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Texas Red