Secondary Antibodies

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:5,000 - 1:20,000.
Reactivities Goat
Conjugation Unconjugated

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation Texas Red

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Texas Red

Applications Flow Cytometry: 1/2,000 - 1/10,000.
Immunofluorescence: 1/500 - 1/2,500.
Reactivities Sheep
Conjugation Texas Red

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:400,000 is suggested for ELISA.
Reactivities Sheep
Conjugation Biotin

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation AP

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:6,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation Biotin

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation AP

Mouse lambda chain rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation HRP

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:40,000.
Western Blot: 1: 500-1:2,000.
Immunohistochemistry: 1:200-1:1,000.
Reactivities Human
Conjugation AP

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation Texas Red

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation TRITC

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation FITC