Secondary Antibodies

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
FLISA: 1/10,000 - 1/50,000
IF Microscopy: 1/1000- 1/5000
Reactivities Goat
Conjugation FITC

Human IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Human
Conjugation TRITC

Rat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
Reactivities Rat
Conjugation Biotin

Goat IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Western Blot/Dot Blot: 1/1,000-1/10,000 or Dot blot.
Immunohistochemistry: 1/500-1/2,500.
ELISA: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

Anti-Goat IgG Antibody Agarose Conjugated

Conjugation Agarose

F(ab')2 Swine IgG (H&L) Antibody Texas Red™ Conjugated

Conjugation Texas Red

F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

Conjugation Phycoerythrin

F(ab')2 Rat IgG (H&L) Antibody Texas Red™ Conjugated

Conjugation Texas Red

F(ab')2 Horse IgG (H&L) Antibody Texas Red™ Conjugated

Conjugation Texas Red

F(ab')2 Rat IgG (H&L) Antibody Rhodamine Conjugated

Conjugation TRITC

F(ab')2 Cat IgG (H&L) Antibody Rhodamine Conjugated

Conjugation TRITC