Secondary Antibodies

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Bovine IgG1 sheep polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG2 sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100 - 1/10000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated