Secondary Antibodies

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26c, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Mouse
Conjugation PE

Human IgD mouse monoclonal antibody, clone IA6-2, PE

Applications Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities Human
Conjugation PE