Secondary Antibodies

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Purified

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Purified

Applications Flow Cytometry: Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / 1 µg/10e6 cells
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells .
ELISA: 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Canine IgE goat polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Western Blot: 1/1000-1/10,000.
Flow Cytometry: 1/200-1/2,000.
This antibody has been reported for use in Immunohistology of Frozen Canine Sections.
Reactivities Canine
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Purified

Applications Flow Cytometry.
Immunoprecipitation. (Ref.1)
Reactivities Chicken
Conjugation Unconjugated

Rabbit IgG mouse monoclonal antibody, clone 2A9, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
FLISA. 
Reactivities Rabbit
Conjugation Unconjugated

Mouse IgG (Rat adsorbed) goat polyclonal antibody, Aff - Purified

Applications ELISA.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Mouse IgM (Mu chain specific), F(ab)2 Fragment rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+.
Immunofluorescence Microscopy: Identification and enumeration of IgM+.
ELISA: < / = 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Low Endotoxin

Applications Flow Cytometry (See Ref.1-4).
Functional Assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Low Endotoxin

Applications Functional Assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Purified

Applications Flow Cytometry. (See Ref.1-5)
Reactivities Mouse
Conjugation Unconjugated