IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified
| Applications | Western blot. Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining. Immunocytochemistry. ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP
| Applications | ELISA: 1/4,000-1/10,000. Western blotting. Dot- and Slot-immunoblotting. Immunohistochemistry on Frozen Sections: 1/10-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 2,750.00
5周
Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified
| Applications | Immunocytochemistry. Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM. Flow cytometry: 1-4 μg/ml. ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free
| Applications | ELISA. FLISA: 1 µg/ml. Western blotting. Dot- and Slot-Immunoblotting Immunocytochemistry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
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