Secondary Antibodies

Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed

Applications WB: >1:10,000
IHC: >1:5,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 550

Mouse IgG1 (Gamma 1 chain) ATTO 550 Conjugated Antibody

Applications WB: >1:10,000
IHC: >1:5,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 550

Mouse IgG2a Antibody ATTO 532 Conjugated Pre-adsorbed

Applications WB: >1:10,000
IHC: >1:5,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 532

Mouse IgG2a Antibody ATTO 550 Conjugated Pre-adsorbed

Applications WB: >1:10,000
IHC: >1:5,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 550

Mouse IgG3 Antibody ATTO 550 Conjugated Pre-adsorbed

Applications WB: >1:10,000
IHC: >1:5,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 550

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Applications WB: User Optimized
IHC: User Optimized
IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified

Applications Immunocytochemistry. 
Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Flow cytometry: 1-4 μg/ml.
ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM.
Reactivities Human
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Immunoblotting.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation Biotin

Human IgM mouse monoclonal antibody, clone M15/8, Purified

Applications Flow Cytometry: 20 µg/ml.
Immunohistochemistry on frozen sections: 20 µg/ml.
ELISA: 5 µg/ml as coating Antibody.
Immunoprecipitation.
Reactivities Human
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation APC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400. 
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Fluorescein Conjugated Mouse IgG (H&L)

Applications WB: User Optimized
IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG1 (Gamma 1 chain) ATTO 647N Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 647N

Mouse IgG2a Antibody ATTO 594 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 594