Secondary Antibodies

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Purified

Applications Flow Cytometry. (See Ref.1-5)
Reactivities Mouse
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26c, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Mouse IgG (Fc specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Texas Red

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Canine IgE goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2,000.
Reactivities Canine
Conjugation FITC

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1/200 - 1/400; Use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/25-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Mouse
Conjugation PE