Secondary Antibodies

Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Rat IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation Texas Red

Rabbit IgG (H&L) Antibody Pre-Adsorbed Texas Red™ Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation Texas Red

Rabbit IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Rabbit IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation Texas Red

Rat IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation TRITC

Rat IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation TRITC

Rat IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation TRITC

Mouse IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation Texas Red

Rabbit IgG F(c) Antibody Texas Red Conjugated

Applications IF: 1:500 - 1:2,500
FC: User Optimized
Conjugation Texas Red

Rat IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:500 - 1:2,500
FC: User Optimized
Conjugation FITC

Rat IgG F(c) Antibody Texas Red™ Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation Texas Red

Mouse IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:500 - 1:2,500
FC: User Optimized
Conjugation FITC

Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation TRITC