Secondary Antibodies

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Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482TC-N

CNY 5,486.00

5周

Mouse IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/60, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488BT-N

CNY 7,161.00

5周

Rabbit IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/10000.
Reactivities Rabbit
Conjugation Biotin
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258AF-N

CNY 5,902.00

5周

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444TC-N

CNY 7,161.00

5周

Monkey IgG (Fab specific) rabbit polyclonal antibody, TRITC

Applications Can be used for direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:40.
Reactivities Monkey
Conjugation TRITC
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490TC-N

CNY 5,486.00

5周

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Can be used to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation TRITC
Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
AP21427FC-N

CNY 5,486.00

5周

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80. 
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC
Mouse IgG (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG-7]
AM32194BT-N

CNY 14,916.00

5周

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Biotin, Purified

Applications Immunoassays.
ELISA: good binding on plastic
Immunohistochemistry on paraffin sections. 
Indirect Immunofluorescence on human peropheral blood cells.
Reactivities Mouse
Conjugation Biotin
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255AF-N

CNY 5,902.00

5周

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252AF-N

CNY 5,902.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Rat IgG1 (gamma 1 chain specific) Mouse Monoclonal Antibody [Clone ID: G1 7E7]
AM08090BT-N

CNY 4,400.00

4周

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin
Rabbit IgG (heavy chain) Rat Monoclonal Antibody [Clone ID: RG-1]
BM499

CNY 7,519.00

4周

Rabbit IgG (heavy chain) rat monoclonal antibody, clone RG-1, Aff - Purified

Applications ELISA.
Immunofluorescence.
Reactivities Rabbit
Conjugation Unconjugated
Human IgG (gamma chain specific) Mouse Monoclonal Antibody [Clone ID: JDC-10]
AM08149BT-N

CNY 3,949.00

4周

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin