Secondary Antibodies

Rat IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antibody can be used:
• As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids.
• In Immunofluorescence and immunoenzyme assay methods.
• For the production of immunoconjugates with a selected marker.
• To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier.
• As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Applications IF: 1:100 - 1:250
FC: 1:100 - 1:250
Reactivities Rat
Conjugation Phycoerythrin

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA: 1/5,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
ELISA.
Second step reagent for rat IgG2b Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE