Secondary Antibodies

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Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252AF-N

CNY 5,902.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
AP31662PU-N

CNY 6,083.00

5周

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Conjugation Unconjugated
Human IgG Rabbit Polyclonal Antibody
AP31649PU-N

CNY 6,083.00

5周

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Conjugation Unconjugated
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020P

CNY 3,500.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Purified

Applications ELISA.
Flow Cytometry: 5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Rat
Conjugation Unconjugated
Chicken IgG / Chicken IgY Mouse Monoclonal Antibody [Clone ID: 4G12]
AM00073PU-N

CNY 4,224.00

5周

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated
Chicken IgG / Chicken IgY Mouse Monoclonal Antibody [Clone ID: 7C2]
AM32197PU-N

CNY 9,338.00

5周

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot  (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation. 
Reactivities Chicken
Conjugation Unconjugated
Chicken IgG / Chicken IgY Mouse Monoclonal Antibody [Clone ID: 7C2]
AM26353PU-N

CNY 6,281.00

5周

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540AF-N

CNY 6,260.00

5周

Canine IgG (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation Unconjugated
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539AF-N

CNY 6,260.00

5周

Canine IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation Unconjugated
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542AF-N

CNY 6,260.00

5周

Canine IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000.
Reactivities Canine
Conjugation Unconjugated
Mouse IgG Rabbit Polyclonal Antibody
AP31662SU-N

CNY 4,565.00

5周

Mouse IgG rabbit polyclonal antibody, Serum

Reactivities Mouse
Conjugation Unconjugated
Human IgG Rabbit Polyclonal Antibody
AP31649SU-N

CNY 4,565.00

5周

Human IgG rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Immunodiffusion.
For Ouchterlony:
Sample Size: 15 µl
Distance between wells: 5 mm
Test time: 4 days at 37°C
Antigens tested: Normal human serum, purified human IgG, normal mouse serum.
Results: Titre with NHS > 1:1000.
Reactivities Human
Conjugation Unconjugated
PrecipHen® (Chicken IgY) Goat Polyclonal Antibody
AP31795AG-N

CNY 6,886.00

5周

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications Immunoprecipitation.
Reactivities Chicken
Conjugation Agarose
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542BT-N

CNY 7,161.00

5周

Canine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Canine
Conjugation Biotin