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Secondary Antibodies
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin
| Applications | Indirect Immunofluorescent staining of IgG+ human B lymphocytes. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry on Frozen Sections. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified
| Applications | Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry on Frozen Sections. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
