Secondary Antibodies

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse
Conjugation Unconjugated

Rabbit IgG (H+L chain) goat polyclonal antibody, Biotin, Lyophilized

Applications Western Blot: 1/5,000-1/20,000 for Western blots.
ELISA: 1/50,000-1/200,000.
Immunohistochemistry: 1/1,000-1/5,000.

Reactivities Rabbit
Conjugation Biotin

Rabbit IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Conjugation ATTO 425

Mouse IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Bovine IgG (H+L chain) goat polyclonal antibody, Agarose

Applications Suitable for antibody purification or to remove unwanted cross-reactivity.
Reactivities Bovine
Conjugation Agarose

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP

Rat IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Conjugation ATTO 488

Biotin Conjugated Rabbit Anti-human IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Human IgG
Conjugation Biotin

Monkey IgA (Secretory component) goat polyclonal antibody, Biotin

Applications Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used in Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Monkey body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/200.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/1,000.
Reactivities Monkey
Conjugation Biotin

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000.
Reactivities Chimpanzee, Monkey
Conjugation Unconjugated

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Conjugation Unconjugated