Secondary Antibodies

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.

Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.

Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgM rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation FITC

Sheep IgG F(c) specific rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Sheep
Conjugation FITC

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
Flow Cytometry: 1:2,000-1:10,000.
Immunofluorescence microscopy: 1:500-1:2,500.
Reactivities Sheep
Conjugation FITC

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation FITC