Secondary Antibodies

Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation HRP

Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000.
Reactivities Sheep
Conjugation HRP

Sheep IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/400,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Sheep
Conjugation HRP

Sheep IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Western Blot (1:1,000- 1:10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1:500- 1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 m g of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(c) specific rabbit polyclonal antibody, HRP

Applications Suitable for Western blotting (1:1,000-1:10,000), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:42,000 of the reconstitution concentration is suggested for this product.
Western blotting: 1:500--1:3,000.
Immunohistochemistry: 1:250-1:1,500.
Reactivities Sheep
Conjugation HRP